Anticancer efficacy of Vitamin D Transport Protein (VDTP) can be ascribed at least to three biological properties of the molecule: 1) activation of tumoricidal macrophages; 2) inhibition of tumor-induced angiogenesis and 3) direct inhibition of cancer cell proliferation, migration and metastatic potential.
Reference: Pacini S, Punzi T, Morucci G, Gulisano M and Ruggiero M: Effects of vitamin D-binding protein-derived macrophage activating factor on human breast cancer cells. Anticancer Res 32: 45-52, 2012
In order to test whether Cytonic proteins (CP) inhibits cancer cells proliferation, we treated MCF-7 cells (cell line derived from human breast cancer) with various concentrations of proteins.
It has been showed that Cytonic proteins directly inhibits breast cancer cells proliferation in dose- dependent manner.
Fig. 1: Breast cancer cells viability after treatment with various concentration of Cytonic proteins (CP). After 72h incubation with Cytonic proteins viability reduction in dose-dependent manner was observed. The effect was the most significant for 400 ng/ml, where cell viability is reduced to ≈ 59.6% with respect to control cells.