Cytonic protein food supplements are pure, mixed isoforms of blended Vitamin D Transport Proteins suspended in 15ml sterile saline solution. One bottle contains approximately 15,000ng Cytonic transport proteins.
Our dropper bottles come with an optional spray attachment. There are approximately 150 drops, or 50 sprays, per 15ml bottle.
1 spray = 300ng
1 drop = 100ng
Recommended daily dose – 1 spray, or 3 drops, once or twice a day.
Vitamin D Transport Protein is a multi-functional plasma protein with many important functions. The most well known one, and the reason for its name, is the transport of vitamin D metabolites. Because VDTP is the primary transporter of vitamin D, it has a role in maintaining the total levels of vitamin D in the body and in regulating the amounts of free (unbound) vitamin D available for specific tissues and cell types to utilise.
Other functions of VDTP are to control bone development, binding of fatty acids, sequestration of actin and a range of less-defined roles in modulating immune and inflammatory responses. The actin removal is considered to be a very important role for VDTP, actins being toxic and released into the body following cell death.
Two amino acids on this protein chain have been shown to be very strongly associated with the activation of macrophages. In some publications, the vitamin D transport protein is shown to respond to enzymes released by inflammation. Studies show that this isoform not only has the potential to activate macrophages, it can turn them off when no longer needed, so can be classed as an immune system regulator.
How to store?
There are 3 options
1. If you do not intend using this right away then it can be frozen for at least one year without loosing activity.
2. If you do intend using it then store it in the coolest part of the fridge, if stored correctly then it will still be very active up to 12 months.
3. Some people wish to go slow with this supplement and maybe spray once a week. In this case it would be prudent to decant some of the solution into a sterile bottle and freeze for later use.
If you wish to travel then keep out of direct sunlight as much as possible and it can travel with the rest of your cosmetics etc. If you wish to take this in a cool bag then this is perfectly okay to do so.
Cytonic Food Supplement – Analytical Parameters
Version: final (audited)
PRODUCT NAME: Cytonic Food Supplement
1. Formulation and characteristics
Formulation and characteristic parameters have been confirmed organoleptically.
2. Protein concentration
Protein concentration of unprocessed VDTP preparation has been determined by Bradford assay (IB-16.05.00).
After processing, VDTP final solutions were prepared in concentrations:
167 ng/ml; 1,000 ng/mI and 3,334 ng/mI (±10%) in relation to initial concentration.
3. Protein identity has been confirmed by mass spectrometry
Accuracy of measurement is ±1%, therefore result of 50,969 Da is acceptable for VDTP (51,243 Da ).
Protein purity has been evaluated with densitometry.
The amounts of 0.5, 1 and 2 ktg of VDTP protein were loaded on SDS-PAGE gel. After electrophoretic separation, gel was stained with Coomassie Brilliant Blue. Absorbance intensity analysis was performed using Image Lab 5.2.1 software.
5. Endotoxin level
Endotoxin level in VDTP has been evaluated using Limulus Amebocyte Lysate (LAL) assay.
Total aerobic microbial count has been evaluated by inoculating Trypone Soya Agar with 100 [..t1 of VDTP and incubation at 37°C for 5 days. No colonies were observed.
Total combined yeast and mold count has been evaluated by inoculating Sabouraud (+ dextrose + chloramfenikol) with 100 µl of VDTP and incubation at 37°C for 5 days. No colonies were observed.
Both tests were done in triplicates.
Absence of E.coli in 10g has been confirmed by inoculating McConkey Broth with 10g of VDTP and incubation at 37°C for 5 days. No turbidity was observed.
Mycoplasma presence in VDTP has been evaluated by NAT (Nucleic Acids Amplification Techniques). Result was negative.
Retrovirus presence in VDTP has been evaluated by PERT (Product Enhanced Reverse Transcriptase assay). Result was negative.
pH of the VDTP solution has been determined using Hanna Instruments HI2212 pH-meter. Measurement was repeated three (3) times and average value was calculated:
Anticancer efficacy of Vitamin D Transport Protein (VDTP) can be ascribed at least to three biological properties of the molecule:
1) activation of tumoricidal macrophages;
2) inhibition of tumor-induced angiogenesis and
3) direct inhibition of cancer cell proliferation, migration and metastatic potential.
Reference: Pacini S, Punzi T, Morucci G, Gulisano M and Ruggiero M: Effects of vitamin D-binding protein-derived macrophage activating factor on human breast cancer cells. Anticancer Res 32: 45-52, 2012
In order to test whether Cytonic proteins (CP) inhibits cancer cells proliferation, we treated MCF-7 cells (cell line derived from human breast cancer) with various concentrations of proteins.
It has been showed that Cytonic proteins directly inhibits breast cancer cells proliferation in dose- dependent manner.
Fig. 1: Breast cancer cells viability after treatment with various concentration of Cytonic proteins (CP). After 72h incubation with Cytonic proteins viability reduction in dose-dependent manner was observed. The effect was the most significant for 400 ng/ml, where cell viability is reduced to ≈ 59.6% with respect to control cells.
Inactive and Active Immune Cells
Immature immune cells are created in our bone marrow, lymph glands and spleen. Then they are released into our blood plasma where they circulate with the blood looking for a specific protein to become fully active and functional immune cells. The protein required to activate all of our immune cells is Vitamin D Transport Protein (VDTP)
In the first 10 seconds of the above video you will see an ‘inactive’ immune cell, it is consuming just enough energy to stay alive but cannot function as a mature immune cells until it is activated.
From 10 seconds onwards you will see different types of active immune cells in action, despite their differences they carry out similar functions and that is to ‘seek and destroy’. We can see the active immune cells using their pseudopods to check red blood cells for any proteins which may indicate an infection. If a ‘suspect’ cell is discovered the the immune cell will consume it then use oxidise bursts to breakdown the infected cell into amino acids and proteins which are recycled into new and healthy red cells.
At 36 seconds we observe an immune cell homing in an a ‘Helmut Cell’ half a red blood cell that served no purpose in the body and therefore must be eliminated and recycled.